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cryogenic grinding yeast samples

For cryogenic disruption cells were ground using a Retsch Haan Germany PM 100 planetary ball mill by the following program 1 g WCW of frozen cells were placed in a 50mL grinding jar with 3 20 mm stainless steel balls all precooled with liquid N 2 and run for three cycles of 3 min 400 rpm cooling with N 2 in between each 3 min

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